Wilms' Tumor 1 (WT1) Mutation Analysis
Methodology
Molecular
Test Description and clinical significance
Genes
WT1
Disease:
Wilms Tumor, acute myeloid leukemia, acute lymphoblastic leukemia, chronic myeloid leukemia, kidney cancer, lung cancer, and skin cancer. Denys-Drash syndrome, Frasier syndrome, congenital nephrotic syndrome, prostate cancer, and WAGR syndrome
Next generation sequencing of gene panel. This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor.
Specimen Requirements
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood, bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated.
CPT(s)
81405
New York Approved
NO
TAT
10 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
