RAF1 single gene test
Methodology
Molecular
Test Description and clinical significance
Genes
RAF1
Disease:
Colon adenocarcinoma, lung adenocarcinoma, bladder urothelial carcinoma, endometrial endometrioid adenocarcinoma, and cutaneous melanoma
Molecular test that utilizes NGS technology to detect mutations in RAF1. Raf-1 proto-oncogene, serine/threonine kinase (RAF1, also known as CRAF) is a gene that encodes a protein that functions downstream of RAS and activates MEK1 and MEK2. Fusions, rearrangements, missense mutations, nonsense mutations, and silent mutations are observed in cancers such as endometrial cancer, intestinal cancer, and stomach cancer.
Specimen Requirements
Collection:
FFPE sections, 5-10 sections are recommended at 4 to 10 micron. A minimum of 20 ?l of RNA with a concentration of at least 5ng/?l and an OD260/280 ratio of 2.0-2.1 respectively, obtained on a spectrophotometer is required.
Stability:
FFPE blocks- Indefinitely/ FFPE Slides-4 weeks
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative. Insufficient specimen (less than 10% of tumor). Unlabeled or improperly labeled specimens. Decalcified specimens. Improperly stored RNA.
Storage & Transport
Store FFPE blocks and slides at room temperature. Genomic RNA must be stored at –60 to –85°C.
CPT(s)
81479, other codes : 81404, 81406
New York Approved
NO
TAT
10 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
