PTEN Single Gene Test
Methodology
Molecular
Test Description and clinical significance
Genes
PTEN (exons 5-6, 9)
Disease:
Glioma, melanoma, prostate, endometrial, breast, ovarian, renal, colon, lung cancers, inherited hamartoma and Cowden syndrome
Deep next generation sequencing of gene panel. PTEN (phosphatase and tensin homolog) is a gene that encodes for phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN. This protein is a lipid/protein phosphatase that plays a role in multiple cell processes, including growth, proliferation, survival, and maintenance of genomic integrity. PTEN acts as a tumor suppressor by negatively regulating the PI3K/AKT signaling pathway. Somatic mutations of PTEN occur in multiple malignancies
Specimen Requirements
Collection:
Specimen should contain atleast 0.5 mL of EDTA, citrate (ACD) or Heparinized bone marrow and peripheral blood specimens
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant.Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood specimens should be kept at room temperature and preferably received within 96 hours of drawing. Samples that are 4 to 7 days old should be refrigerated
CPT(s)
81321 if full sequence analysis & 81322 if Known familial variant & 81323 if duplication/deletion variant
New York Approved
NO
TAT
7 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
