Liver & Biliary Cancers 50 Genes NGS Panel
Methodology
Molecular
Test Description and clinical significance
Genes
DNA HOTSPOT GENES (44)
AKT1, AKT2, ALK, AR, ARAF, BRAF, CDK4, CDKN2A, CHEK2 CTNNB1, EGFR, ERBB2, ERBB3, ERBB4, ESR1, FGFR1, FGFR2, FGFR3 FGFR4, FLT3, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, NTRK1, NTRK2, NTRK3 PDGFRA, PIK3CA, PTEN, RAF1, RET, ROS1, SMO, TP53
CNV GENES (14)
ALK, AR, CD274, CDKN2A, EGFR, ERBB2, ERBB3, FGFR1, FGFR2
FGFR3, KRAS, MET, PIK3CA, PTEN
FUSION DRIVER GENES (20)
ABL1, ALK, AXL, BRAF, EGFR, ERBB2, ETV4, ETV5, FGFR1
FGFR2, FGFR3, MET, NTRK1, NTRK2, NTRK3, PDGFRA, PPARG, RAF1
RET, ROS1
Disease:
Biliary and liver cancers
Deep next generation sequencing of gene panel for biliary and liver tumor associated mutation detection
Specimen Requirements
Collection:
FFPE sections, 5-10 sections are recommended at 4 to 10 micron. A minimum of 20 ?l of each DNA and RNA with a concentration of at least 5ng/?l and an OD260/280 ratio of 1.7-1.9 and 2.0-2.1 respectively, obtained on a spectrophotometer is required.
Stability:
FFPE blocks- Indefinitely/ FFPE Slides-4 weeks
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative. Insufficient specimen (less than 10% of tumor). Unlabeled or improperly labeled specimens. Decalcified specimens. Improperly stored DNA or RNA.
Storage & Transport
Store FFPE blocks and slides at room temperature. Genomic DNA must be stored at 2–8°C for several weeks post extraction, then at –15 to –25°C for long term storage and RNA must be stored at –60 to –85°C.
CPT(s)
81455
New York Approved
YES
TAT
4-5 days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
