Concurrent DeepSight™ on Liquid + siPortfolio Multi-Omics™ on Tissue
Methodology
Molecular
Test Description and clinical significance
Disease:
Solid tumors
On Tissue: siPortfolio Multi-Omics™
• NGS (500+ genes, TMB, MSI)
• HRD for PARPi therapy
• IHC, FISH (as clinically pertinent and necessary)
On Liquid: DeepSight™ Comprehensive 523 Genes
including TMB and MSI
Specimen Requirements
Collection:
FFPE sections, 5-10 sections are recommended at 4 to 10 micron. A minimum of 20 ?L of each DNA with a concentration of at least 5 ng/?L and an OD260/280 ratio of 1.7-1.9, obtained on a spectrophotometer is required.
Two-Three tubes of peripheral blood (10mL/tube) are collected in PAXgene Blood ccfDNA, For best results, a 21G or 22G needle is advised. Previously extracted cfDNA is acceptable. A minimum of 50 µL of each cfDNA with a
concentration of at least 0.60 ng/µ, corresponding to total at least 30ng of cfDNA,
fragment size between 100-200 bp, obtained on a TapeStation Cell-free DNA ScreenTape
or related instruments for assessment of cfDNA quality.
Stability:
FFPE blocks- Indefinitely/ FFPE Slides-4 weeks
for Liquid biopsy: 48 hrs.
Unacceptable Conditions:
Tissue: Specimen submitted is in the incorrect fixative. Insufficient specimen (less than 10% of tumor). Unlabeled or improperly labeled specimens. Decalcified specimens. Improperly stored DNA.
Liquid biopsy: Do not freeze.
Specimen submitted is not in the PAXgene Blood ccfDNA tube or in the PAXgene Blood
ccfDNA tube after expired date.
Storage & Transport
Store FFPE blocks and slides at room temperature. Slides may be stored at ambient temperature for up to 4 weeks prior to DNA extraction. Genomic DNA must be stored at 2–8°C for several weeks post extraction, then at –15 to –25°C for long term.
Store tubes within the recommended temperature range (18°C to 25°C) within 2 days, including shipping.
CPT(s)
"81462, 81455, 88381 and G0452(26) *Additional CPT codes may be applied"
New York Approved
No
TAT
7-10 days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
